UltraNuclease GMP-grade (250 U/μL)

Description: DescriptionUltraNuclease is a genetically engineered endonuclease from Serratia marcescens. The enzyme digests all forms of DNA/RNA moleculars into oligonucleotides of about 5 bp. The product is expressed in Escherichia coli (E. coli) and purified under the GMP environments. The nuclease is equivlent to benzonase in reducing host nucleic acid residues to pg-grade, improving the performance and safety of biological products of applications including virus purification, vaccine manufacturing, and protein/polysaccharide pharmaceutical manufacturing. It can also used in protein study to reduce the viscosity of cell supernatant and cell lysate, increase protein purification efficiency and enhance protein functional research.UltraNuclease is provided in the form of a sterilized reagent, eluted in buffer (20 mM Tris-HCl pH 8.0, 2 mM MgCl2, 20 mM NaCl, 50% glycerin), with the appearance of a colorless, transparent liquid. This product is produced by GMP process requirements and provided in a liquid form.FeatureWide range of applications: degrade all forms of DNA and RNAHigh purity and activity: purity ≥ 99%; specific activity ≥ 1.5×106 U/mgStrong adaptability: strong tolerance, adapt to a variety of reaction conditionsComply with pharmacopeia: no animal origin; no antibiotics; lower endotoxinQualification: product gets the FDA DMF filling which can shorten the customers for new drug applicationGMP-grade manufacturing: strict standards to meet the needs of large-scale use for research to manufactureApplicationPurification of viral vaccines, viral vectors for the vaccine, and oncolytic viruses, removing DNA/RNA from proteins and other biologicalsReduction of viscosity caused by nucleic acidsSample preparation in electrophoresis and chromatographyPrevention of cell clumpingSpecificationExpression HostRecombinant E. coli with UltraNuclease geneMolecular Weight26.5 kDaIsoelectric point6.85Purity≥ 99% (SDS-PAGE)Storage Buffer20 mM Tris-HCl pH8.0, 2 mM MgCl2, 20 mM NaCl, 50% glycerinUnit DefinitionThe definition of one Activity unit (U) is the amount of enzyme used to change the absorption value of △A260 by 1.0 in 30 minutes in a 2.625 mL reaction system at 37°C with a pH of 8.0 (equivalent to complete digestion of 37 μg salmon sperm DNA into oligonucleotides).ComponentsComponents No.Name20157ES25 (25 KU)20157ES60 (100 KU)20157ES90 (5 MU)20157UltraNuclease GMP-grade (250 U/μL)100 μL400 μL20 mLShipping and StorageThe product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for two years. If the product is opened and has been stored at 4℃ for more than a week, we recommend filtering the product to prevent microbial contamination.

Price: 284.99 USD

Location: Camarillo, California

End Time: 2024-10-12T06:10:49.000Z

Shipping Cost: 0 USD